منابع مشابه
Human gastric lipase: a sulfhydryl enzyme.
One sulfhydryl group was modified per mol of native human gastric lipase after incubation at pH 8.0 with 5,5'-dithiobis(2-nitrobenzoic acid) for 18 h or with 4,4'-dithiopyridine for 100 min. With both reagents a direct correlation was found between the modification of one sulfhydryl group and the loss of human gastric lipase activity. Incubation of human gastric lipase with a new hydrophobic su...
متن کاملChanges in Enzyme Efficiency During Lipase-Catalyzed Hydrolysis of Canola Oil in a Supercritical Bioreactor
Enzyme efficiency was investigated in the lipase-catalyzed hydrolysis of canola oil in supercritical carbon dioxide (SCCO2). Immobilized lipase from Mucor miehie (Lipozyme IM) was used as the catalyst and the results showed that enzyme efficiency dropped at high pressures indicating a possible change in enzyme microstructure. Therefore, scanning electron microscopy (SEM) was used...
متن کاملFunctional characterization of a chimeric lipase genetically engineered from human lipoprotein lipase and human hepatic lipase.
Lipoprotein lipase (LPL) and hepatic lipase (HL) mediate the hydrolysis of triglycerides and phospholipids present in circulating lipoprotein particles and are essential for normal lipid metabolism. Both enzymes have a similar primary amino acid structure and share requirements for intact catalytic, lipid binding, and heparin binding domains. However, LPL and HL exhibit different substrate spec...
متن کاملA double blind lipase for lipase comparison of a high lipase and standard pancreatic enzyme preparation in cystic fibrosis.
A standard acid resistant microsphere pancreatic enzyme preparation was compared with identical capsules half filled with mini-tablets of a new high lipase preparation in a randomised double blind crossover study in children with cystic fibrosis. Each patient received his/her usual number of capsules and the same dose of lipase during each period of the study. Eighteen patients completed the st...
متن کاملOn the mechanism of enzyme inhibition by sulfhydryl reagents.
In recent years a great deal of evidence has accumulated to show that intact -SH groups are necessary for the full activity of a variety of enzymes. The exact function of the thiol group in the protein part of these “sulfhydryl enzymes,” however, remains obscure. It has been suggested that the -SH group directly participates in the activity of these enzymes, and that it might be actually locate...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1988
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69185-9